Part:BBa_K4175010:Design

IL-6R-Notch-Gal4KRAB

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 60
  Illegal XbaI site found at 271
  Illegal PstI site found at 55
  Illegal PstI site found at 1117
  Illegal PstI site found at 1294
  Illegal PstI site found at 1519
  Illegal PstI site found at 1560
  Illegal PstI site found at 1606
  Illegal PstI site found at 2638
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 60
  Illegal PstI site found at 55
  Illegal PstI site found at 1117
  Illegal PstI site found at 1294
  Illegal PstI site found at 1519
  Illegal PstI site found at 1560
  Illegal PstI site found at 1606
  Illegal PstI site found at 2638
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 60
  Illegal BamHI site found at 76
  Illegal BamHI site found at 1194
  Illegal XhoI site found at 19
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 60
  Illegal XbaI site found at 271
  Illegal PstI site found at 55
  Illegal PstI site found at 1117
  Illegal PstI site found at 1294
  Illegal PstI site found at 1519
  Illegal PstI site found at 1560
  Illegal PstI site found at 1606
  Illegal PstI site found at 2638
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 60
  Illegal XbaI site found at 271
  Illegal PstI site found at 55
  Illegal PstI site found at 1117
  Illegal PstI site found at 1294
  Illegal PstI site found at 1519
  Illegal PstI site found at 1560
  Illegal PstI site found at 1606
  Illegal PstI site found at 2638
  Illegal NgoMIV site found at 1432
  Illegal NgoMIV site found at 2423
  Illegal NgoMIV site found at 2558
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence has been codon optimized for human.

Source

human extracellular IL-6R (BBa_K4175002), Notch core (BBa_K4175001), ZF_GAl4_KRAB (BBa_K2446037)